Germ line development: lessons learned from pluripotent stem cells

Martinez-Arroyo, A M, Medrano, J V, Remohi, J, Simon, C,
Curr Opin Genet Dev. Oct. 2014 doi: 10.1016/j.gde.2014.09.011


Current knowledge about mammalian germ line development is mainly based on the mouse model and little is known about how this fundamental process occurs in humans. This review summarizes our current knowledge of genetic and epigenetic germ line development in mammals, mainly focusing on primordial germ cell (PGC) specification events, comparing the differences between mouse and human models. We also emphasize the knowledge derived from the most successful strategies used to generate germ cell-like cells in vitro in both models and major obstacles to obtaining bona fide in vitro-derived gametes are considered.